학회지 리뷰
Korean Journal of Microbiology (2022) Vol. 58, No. 1, pp. 1-11
DOI https://doi.org/10.7845/kjm.2022.2007
In vitro effects of cigarette smoke condensate on Fusobacterium nucleatum subsp. nucleatum
Cheul Kim1, Jae-Hyung Lee2, Seok Bin Yang2, Hee-Su Lee1, and Ji-Hoi Moon2*
1Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea
2Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
DOI https://doi.org/10.7845/kjm.2022.2007
In vitro effects of cigarette smoke condensate on Fusobacterium nucleatum subsp. nucleatum
Cheul Kim1, Jae-Hyung Lee2, Seok Bin Yang2, Hee-Su Lee1, and Ji-Hoi Moon2*
1Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea
2Department of Oral Microbiology, College of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
Fusobacterium nucleatum has been reported to be more abundant in subgingival microbiome of smokers than that of non-smokers. This study investigated in vitro effect of cigarette smoke condensate (CSC) on F. nucleatum subsp. Nucleatum ATCC 25586. Sterile CSC-rich broth (400 ml) was prepared with smoke derived from 40 burning cigarettes (8 mg tar and 0.7 mg nicotine/cigarette). The total growth of F. nucleatum for 24 h decreased in the presence of CSC (6.25–25%). Whereas the relative biofilm formation, auto-aggregation activity, and resistance to erythromycin increased in the presence of CSC. Genome-wide transcriptome analysis revealed that a total of 163 genes were differentially expressed more than 2-fold by CSC. Most of these were functionally related to transport and metabolism of inorganic ion and amino acid, and energy production and conversion. Upregulation of the gene encoding macrolide-efflux protein was also identified. The genes most prominently upregulated by CSC were associated with iron acquisition and use of glutamate and tryptophan, all of which can directly affect the transition of F. nucleatum to a biofilm state. Meanwhile, the genes related to utilization of tyrosine and serine were downregulated. Collectively, CSC induced phenotypic changes of F. nucleatum subsp. nucleatum, such as decreased cell division, transition to a biofilm state and reduced susceptibility to some antibiotics. These phenotypic changes seem to be partly related to altered expression of genes associated with iron utilization and amino acid preference. Our results provide new clues into the role of F. nucleatum in the oral cavity of smokers.
Korean Journal of Microbiology (2022) Vol. 58, No. 2, pp.61-108
DOI https://doi.org/10.7845/kjm.2022.2031
Microbiome analysis of sponges Callyspongia ramosa and Callyspongia confoederata
Youjin Kim1†, Kyoung-Ho Kim2†, Junpyo Lee1, and Jin-Sook Park1*
1Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34430, Republic of Korea
2Department of Microbiology, Pukyong National University, Pusan 48513, Republic of Korea
DOI https://doi.org/10.7845/kjm.2022.2031
Microbiome analysis of sponges Callyspongia ramosa and Callyspongia confoederata
Youjin Kim1†, Kyoung-Ho Kim2†, Junpyo Lee1, and Jin-Sook Park1*
1Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34430, Republic of Korea
2Department of Microbiology, Pukyong National University, Pusan 48513, Republic of Korea
Diversities of prokaryotic symbionts were investigated in two sponge species, Callyspongia ramosa and Callyspongia confoederata collected from Jeju Island by 16S rRNA gene amplicon sequencing using. The V3-V4 and V5-V6 regions of the 16S rRNA gene of bacteria and archaea were amplified and sequenced using Illumina MiSeq platform. Gammaproteobacteria (85.40–87.50%), Bacteroidetes (8.51–10.71%), and Alphaproteobacteria (0.98–1.15%) were major taxa in C. ramose while Gammaproteobacteria (25.06–40.05%), Bacteroidetes (26.99–27.38%), Tenericutes (8.55–16.20%), Betaproteobacteria (8.17–9.51%), Alphaproteobacteria (7.35–8.46%), Cyanobacteria (3.47–10.35%), Chloroflexi (0.51–1.71%), and Actinobacteria (0.45–1.18%) in C. confoederata. Prokaryotic diversity of C.confoederata was more complex than that of C. ramosa and unique prokaryotic communities differentiated the different species in the same genus. This study would provide additional evidence that the sponge symbiotic communities have hostspecificity and elucidate their diversity in detail.